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Working solutions were prepared by appropriate dilution in acetonitrile:benzene. The extraction through cartridges, C 18 Sep-Pak, was time consuming, lengthening the sample preparation. The problem was solved by increasing the quantity of hot water added to thin the milk from 20 to 40mL. This way, the sample preparation became faster. Aflatoxins M 1 and M 2 were extracted from warm, diluted milk on a C 18 Sep-Pak cartridge, eluted with ether through a silica column, eluted with dichloromethane: alcohol, and derivatized with TFA.
Liquid chromatography peaks were detected fluorometrically and compared with the standard-TFA derivative. All samples analyzed were within their expiry date. The linearity of the method was carried out in the range of 0.
The precision of the method was calculated as the relative standard deviation RSD. The detection and quantitation limit were determined based on the standard deviation of the response and the slope The obtained show the chromatograms after derivatization with TFA, a of goat milk free from aflatoxins M 1 and M 2b of a sample spiked with aflatoxins M 1 and M 2 and c of a naturally contaminated sample Figure 1. The quantification limit was established as the lowest concentration of an analyte, using an appropriate standard or sample, could be determined with an acceptable level of accuracy and precision The obtained in our laboratory, during the method validation, demonstrated that this method is efficient to analyze aflatoxins M 1 and M 2 in goat milk samples with good precision, accuracy.
The limit of detection and quantification are low enough to be used to analyze aflatoxins M 1 and M 2 in small quantities. In addition to that, sample preparation is adequate to obtain an extract without interfering peaks, as we can see in the non-contaminated goat milk chromatogram Figure 1.
The Table 1 shows the of the levels of aflatoxins M 1 found in the samples analyzed. The were compared with more regulations strict like the one adopted by the European Union. One 2.
Four samples of pasteurized milk There are several studies on the incidence of aflatoxins in cow milk in different countries like Korea 15Colombia 16Argentina 17Greece 18Brazil and so on, but surveys on the occurrence of aflatoxins in goat milk are rare. Landscape Ecology 1 1Trap-nest bore diameter preferences among sympatric Passaloecus spp.
Brazil Demographics. Great Lakes Entomology 24, Fye, R. The effect of forest disturbances on populations of wasps and bees in northwestern Ontario Hymenoptera: Aculeata. The Canadian Entomologist 10Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological economics 68 3Foraging ranges of solitarybees.
Journalof Animal Ecology 71, — Food availability affects Osmia pumila Hymenoptera: Megachilidae foraging, reproduction, and brood parasitism. Oecologia 4Polistes wasps Hymenoptera: Vespidae as control agents for lepidopterous cabbage pests.
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